4S36
Crystal structure of the C-terminal domain of R2 pyocin membrane-piercing spike
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 46.205, 46.205, 144.954 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.015 - 1.460 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.480 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.036 | 0.589 |
| Number of reflections | 16695 | |
| <I/σ(I)> | 59.3 | 5.5 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 27 | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 34% PEG 400, 200mM NaSO4, 100mM Na acetate, pH 5.0, 15mg/ml protein, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
