4S2W
Structure of E. coli RppH bound to sulfate ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.391, 38.851, 56.806 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 19.425 - 1.992 |
| R-factor | 0.1724 |
| Rwork | 0.170 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 1.992 | 2.000 |
| Rmerge | 0.090 | 0.523 |
| Number of reflections | 10978 | |
| <I/σ(I)> | 2.87 | |
| Completeness [%] | 93.2 | 84.7 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.4 M ammonium sulfate, 12% PEG 3350, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
