4S2S
Crystal Structure of Fab fragment of monoclonal antibody RoAb13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-15 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.200, 88.640, 78.450 |
| Unit cell angles | 90.00, 103.19, 90.00 |
Refinement procedure
| Resolution | 15.402 - 2.100 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hil |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.203 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.450 | 2.210 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 55876 | |
| Completeness [%] | 95.4 | 89.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 2000 monomethyl ether, 0.01M nickel chloride, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
