4S1N
The crystal structure of phosphoribosylglycinamide formyltransferase from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.206, 46.227, 91.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.300 - 2.700 |
| R-factor | 0.2041 |
| Rwork | 0.200 |
| R-free | 0.27580 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.485 |
| Data reduction software | DENZO (Builder/HKL3000) |
| Data scaling software | SCALEPACK (Builder/HKL3000) |
| Phasing software | SHELXD (Builder/HKL3000) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.142 | 0.690 |
| Number of reflections | 5384 | |
| <I/σ(I)> | 14.03 | 2.57 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 9.6 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.16M Calcium Acetate, 0.08M Sodium Cacodylate:HCl, 14.4%(w/v) PEG8000, 20% (v/v)Glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
