4S17
The crystal structure of glutamine synthetase from Bifidobacterium adolescentis ATCC 15703
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 134.052, 134.052, 298.915 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.780 - 2.300 |
| R-factor | 0.2409 |
| Rwork | 0.238 |
| R-free | 0.28740 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.588 |
| Data reduction software | DENZO (Builder/HKL3000) |
| Data scaling software | SCALEPACK (Builder/HKL3000) |
| Phasing software | SHELXD (Builder/HKL3000) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.112 | 0.741 |
| Number of reflections | 137577 | |
| <I/σ(I)> | 14.9 | 2.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M MgCl2, 0.1M HEPES:NaOH, 30% (v/v) PPG P400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
