4RZK
Crystal structure of Sulfolobus solfataricus Hsp20.1 ACD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | H 3 |
| Unit cell lengths | 91.124, 91.124, 74.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.915 - 2.677 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 6449 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 2% v/v tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 16% w/v polyethylene glycol 3,350, VAPOR DIFFUSION, temperature 293.0K |
