4RZJ
Structure of the complex of type 1 ribosome inactivating protein from Momordica balsamina with N-acetylglucosamine at 1.98 Angstrom resolution using crystals grown in different conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-11-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 |
| Unit cell lengths | 130.148, 130.148, 39.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.070 - 1.980 |
| R-factor | 0.17171 |
| Rwork | 0.168 |
| R-free | 0.21877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4QGJ |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.626 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.080 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 15675 | |
| <I/σ(I)> | 29.13 | 1.5 |
| Completeness [%] | 100.0 | 85.4 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 100mM sodium phosphate, 14% PEG 6000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
