4RZ7
Crystal Structure of PVX_084705 with bound PCI32765
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.556, 117.422, 68.219 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 47.936 - 2.351 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.105 | 0.036 | 0.729 |
| Number of reflections | 62654 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 99.9 | 99.1 | 99.9 |
| Redundancy | 3.7 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | PEG 8000, Tris 8.5, VAPOR DIFFUSION, temperature 293K |
