4RZ0
Crystal Structure of Plasmodium falciparum putative histone methyltransferase PFL0690c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.730, 53.474, 111.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.223 - 2.487 |
| R-factor | 0.2225 |
| Rwork | 0.220 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.336 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.487 | 6.780 | 2.500 |
| Rmerge | 0.097 | 0.041 | 0.840 |
| Number of reflections | 10658 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 99.4 | 99.4 | 94.6 |
| Redundancy | 7.6 | 6.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20% PEG3350, 0.2M NH4Cl, VAPOR DIFFUSION, temperature 293K |
