4RYW
Crystal structure of the photoconverted green fluorescent protein NowGFP_conv (the variant of cyan Cerulean) at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.132, 98.021, 71.671 |
| Unit cell angles | 90.00, 111.63, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.2097 |
| Rwork | 0.205 |
| R-free | 0.29530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.509 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.117 | 0.057 | 0.502 |
| Number of reflections | 23175 | ||
| <I/σ(I)> | 6.6 | ||
| Completeness [%] | 100.0 | 100 | 99.9 |
| Redundancy | 3.7 | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Na Acetate pH=4.5, 30% (v/v) PEG 5000 mono methyl ether, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
