4RYV
Crystal structure of yellow lupin LLPR-10.1A protein in complex with trans-zeatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-29 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.970, 63.910, 47.560 |
| Unit cell angles | 90.00, 126.68, 90.00 |
Refinement procedure
| Resolution | 42.490 - 1.380 |
| R-factor | 0.14274 |
| Rwork | 0.142 |
| R-free | 0.17013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1icx |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.987 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (10.2.31) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.054 | 0.209 |
| Number of reflections | 34754 | |
| <I/σ(I)> | 24.74 | 6.48 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 1.8 M (NH4)2SO4, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
