4RYS
Crystal structure of the green fluorescent rotein NowGFP (the variant of cyan Cerulean) at pH 4.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.589, 51.274, 60.997 |
| Unit cell angles | 90.00, 108.28, 90.00 |
Refinement procedure
| Resolution | 26.190 - 1.180 |
| R-factor | 0.1318 |
| Rwork | 0.132 |
| R-free | 0.14250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.029 |
| RMSD bond angle | 3.156 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.220 |
| High resolution limit [Å] | 1.180 | 2.540 | 1.180 |
| Rmerge | 0.057 | 0.037 | 0.425 |
| Number of reflections | 107191 | ||
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 99.5 | 99.4 | 96.8 |
| Redundancy | 3.6 | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 14mM KH2PO4, pH 4.8, 14% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
