4RXL
Crystal structure of Molybdenum ABC transporter solute binding protein Vc_A0726 from Vibrio Cholerae, Target EFI-510913, in complex with tungstate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-21 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.445, 56.633, 50.146 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.520 |
| R-factor | 0.17245 |
| Rwork | 0.171 |
| R-free | 0.20998 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.312 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.124 | 0.870 |
| Number of reflections | 28440 | |
| <I/σ(I)> | 18.4 | 2.5 |
| Completeness [%] | 94.0 | 100 |
| Redundancy | 7.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | PROTEIN (10 MM HEPES, PH 7.5, 5 MM DTT, 10 MM SODIUM TUNGSTATE); RESERVOIR: 0.1 M TRIS:HCL, PH 7.0, 0.2 M MAGNESIUM CHLORIDE, 10% PEG8000; CRYOPROTECTION: RESERVOIR SOLUTION PLUS 20% GLYCEROL (VOL/VOL); VAPOR DIFFUSION, SITTING DROP, temperature 294K |
