Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.645, 43.564, 65.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.200 - 1.250 |
| R-factor | 0.1234 |
| Rwork | 0.122 |
| R-free | 0.14940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dmx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.533 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.200 | 40.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 3.390 | 1.250 |
| Rmerge | 0.048 | 0.019 | 0.413 |
| Number of reflections | 23934 | ||
| <I/σ(I)> | 10.9 | 2.04 | |
| Completeness [%] | 92.5 | 99.4 | 59.3 |
| Redundancy | 6.1 | 6.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.4 M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
