4RWM
Kuenenia stuttgartiensis hydroxylamine oxidoreductase cryoprotected with ethylene glycol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 130.290, 130.290, 130.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.14776 |
| Rwork | 0.147 |
| R-free | 0.16445 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.480 |
| Number of reflections | 68117 | |
| <I/σ(I)> | 19.6 | 3.9 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1.3 M ammonium sulfate, 0.05- 0.1 M sodium phosphate buffer, pH 7.4 and the detergent additive cyclohexylbutanoyl-N-hydroxyethylglucamide (C-HEGA-10) at a final concentration of 35 mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
