4RWH

Crystal structure of T cell costimulatory ligand B7-1 (CD80)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	I 41
Unit cell lengths	54.001, 54.001, 85.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.534 - 1.802
R-factor	0.1877
Rwork	0.187
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dr9
RMSD bond length	0.006
RMSD bond angle	0.967
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.534
High resolution limit [Å]	1.802
Number of reflections	11322
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	2.4M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K