4RWB
Racemic influenza M2-TM crystallized from monoolein lipidic cubic phase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21/c 1 |
| Unit cell lengths | 40.930, 41.230, 27.930 |
| Unit cell angles | 90.00, 95.68, 90.00 |
Refinement procedure
| Resolution | 19.390 - 2.000 |
| R-factor | 0.2816 |
| Rwork | 0.281 |
| R-free | 0.29560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lbw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.641 |
| Data reduction software | XPREP (2008/2 for Windows) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.390 | 19.390 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.520 | 2.000 |
| Number of reflections | 6119 | 311 | 164 |
| <I/σ(I)> | 8.88 | 17.64 | 2.27 |
| Completeness [%] | 96.2 | 94.8 | 95.9 |
| Redundancy | 7.14 | 6.73 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | monoolein lipidic cubic phase | 6.5 | 298 | Peptide combined with monoolein at 15-20% (w/w) by codissolving in trifluoroethanol, LCP generated by mixing vacuum-dried solid components with water at 60:40 lipid:water ratio. 200 nL LCP bolus combined with 1 uL solution containing 0.1 M ADA pH 6.5, 24% MPD in glass sandwich plates. Crystals grew in less than 1 day. , monoolein lipidic cubic phase, temperature 298K |
