4RW1
Hen egg-white lysozyme structure from a spent-beam experiment at LCLS: original beam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2012-11-01 |
| Detector | CSPAD |
| Wavelength(s) | 1.63 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.240, 79.240, 38.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.050 - 1.900 |
| R-factor | 0.22085 |
| Rwork | 0.219 |
| R-free | 0.25575 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4et8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | REFMAC (5.5.0102) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 10048 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 3 | 293 | After crystallization, the solution used for growing the crystals (20 % NaCl, 6 % PEG 6000, 1 M Na acetate pH 3.0) was exchanged for storage solution (10 % NaCl, 1.0 M Na acetate pH 3.4), batch, temperature 293K |
