4RVH

Crystal structure of MtmC in complex with SAH and TDP-4-keto-D-olivose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-26
Detector	RAYONIX MX300HS
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	135.359, 135.359, 127.307
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.400
R-factor	0.2056
Rwork	0.204
R-free	0.23114
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.011
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.400
Number of reflections	21204

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.1 M MES, pH 5.5, 0.2 M ammonium acetate, 16% PEG 4000, 1 mM SAH, 1 mM TDP-4-keto-D-olivose, VAPOR DIFFUSION, HANGING DROP, temperature 295K