4RV9

Crystal structure of MtmC in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Detector technology	CCD
Collection date	2014-06-26
Detector	RAYONIX MX300HS
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	134.245, 134.245, 130.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.200
R-factor	0.20523
Rwork	0.203
R-free	0.24114
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e2x
RMSD bond length	0.006
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	28770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.1 M MES pH 5.5, 0.2 M ammonium acetate, 16% PEG 4000, 1 mM SAH, VAPOR DIFFUSION, HANGING DROP, temperature 295K