4RV5
The crystal structure of a solute-binding protein from Anabaena variabilis ATCC 29413 in complex with pyruvic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.525, 100.588, 150.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.730 - 1.040 |
| R-factor | 0.1127 |
| Rwork | 0.112 |
| R-free | 0.13060 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.232 |
| Data reduction software | DENZO (Builder/HKL3000) |
| Data scaling software | SCALEPACK (Builder/HKL3000) |
| Phasing software | SHELXD (Builder/HKL3000) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.800 | 1.060 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.046 | 0.569 |
| Number of reflections | 344461 | |
| <I/σ(I)> | 31.1 | 1.98 |
| Completeness [%] | 97.2 | 81.9 |
| Redundancy | 5.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M MaCl2, 0.1M Bis-Tris:HCl, 25% (w/v) PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
