4RUS
Carp Fishelectin, holo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.071560 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 204.410, 120.810, 69.270 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 29.670 - 1.700 |
| R-factor | 0.1955 |
| Rwork | 0.195 |
| R-free | 0.21372 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ruq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.151 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.098 | 0.351 |
| Number of reflections | 171485 | |
| Completeness [%] | 93.2 | 95.1 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M N-Acetyl glucosamine, 19% PEG 3350, 0.2 M magnesium formate, 0.05 M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
