4RU3
Crystal structure of the cell puncturing protein Orf41 from Pseudomonas phage SN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 84.540, 84.540, 84.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.513 - 1.172 |
| R-factor | 0.1348 |
| Rwork | 0.134 |
| R-free | 0.14990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.513 | 1.240 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.068 | 0.293 |
| Number of reflections | 67579 | |
| <I/σ(I)> | 23.1 | 8.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.7 | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 291 | 21% PEG 10K, 0.1M Ammonium acetate, 0.1M Bis-Tris pH 5.0, VAPOR DIFFUSION, temperature 291K |
