4RTH
The crystal structure of PsbP from Zea mays
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.26 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.058, 38.328, 61.116 |
| Unit cell angles | 83.41, 66.40, 63.53 |
Refinement procedure
| Resolution | 32.090 - 1.600 |
| R-factor | 0.17086 |
| Rwork | 0.170 |
| R-free | 0.19294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v2b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.030 | 0.131 |
| Number of reflections | 37230 | |
| <I/σ(I)> | 44.3 | |
| Completeness [%] | 94.8 | 90.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2M tri-Ammonium Citate PH7.0, 20% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
