4RTF
Crystal structure of molecular chaperone DnaK from Mycobacterium tuberculosis H37Rv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 117.866, 117.866, 134.943 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.770 |
| R-factor | 0.21096 |
| Rwork | 0.208 |
| R-free | 0.26881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v7y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.092 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.820 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 14714 | |
| <I/σ(I)> | 52.8 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 21.4 | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M Ammonium Chloride, 0.1 M HEPES, 20% PEG 6000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
