4RTE
The X-ray structure of bovine pancreatic ribonuclease incubated in the presence of an excess of cisplatin (1:10 ratio)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.770, 63.770, 63.575 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.230 - 1.950 |
| R-factor | 0.16986 |
| Rwork | 0.167 |
| R-free | 0.22389 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1RNX |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.896 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.230 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.090 |
| Number of reflections | 11243 |
| Completeness [%] | 99.8 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | protein concentration 15 mg mL-1 and a precipitant solution containing 30 % ammonium sulfate and 3.0 M sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
