4RT6
Structure of a complex between hemopexin and hemopexin binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979110 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 45.060, 348.860, 77.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.430 - 2.800 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 4RM6 1qhu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.760 | 2.970 |
| High resolution limit [Å] | 2.800 | 3.430 | 2.800 |
| Number of reflections | 30025 | ||
| Completeness [%] | 96.3 | 99.5 | 85.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 100mM citric acid, 25% w/v PEG 4000, 200mM ammonium sulfate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
