4RSO

The structure of the neurotropic AAVrh.8 viral vector

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.91860
Spacegroup name	P 21 21 21
Unit cell lengths	355.500, 358.900, 371.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.500
R-factor	0.217
Rwork	0.217
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AAV8 VP1
RMSD bond length	0.008
RMSD bond angle	1.370
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.500	7.530	3.500
Number of reflections	487416
<I/σ(I)>	3.2
Completeness [%]	90.0	97.4	86
Redundancy	2.8	3.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	Crystallization drops consisted of virus sample (10 mg/ml) in 1X PBS mixed with reservoir solution (5.5% PEG8000 in 1xPBS) at a 1:1 ratio (v/v) and equilibrated against 1 ml of reservoir solution at 298K. pH 7.4; temperature 298K; hanging-drop vapor-diffusion, VAPOR DIFFUSION, HANGING DROP