4RSO
The structure of the neurotropic AAVrh.8 viral vector
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 355.500, 358.900, 371.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.500 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AAV8 VP1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 7.530 | 3.500 |
| Number of reflections | 487416 | ||
| <I/σ(I)> | 3.2 | ||
| Completeness [%] | 90.0 | 97.4 | 86 |
| Redundancy | 2.8 | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | Crystallization drops consisted of virus sample (10 mg/ml) in 1X PBS mixed with reservoir solution (5.5% PEG8000 in 1xPBS) at a 1:1 ratio (v/v) and equilibrated against 1 ml of reservoir solution at 298K. pH 7.4; temperature 298K; hanging-drop vapor-diffusion, VAPOR DIFFUSION, HANGING DROP |
