4RSL

Structure of fructosyl peptide oxidase from E. terrenum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	65.603, 80.018, 83.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.873 - 1.900
R-factor	0.1928
Rwork	0.191
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.134
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.968
High resolution limit [Å]	1.900	1.900
Number of reflections	35108
Completeness [%]	99.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.2	298	0.2M potassium phosphate dibasic, 20%(w/v) polyethylene glycol 3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K