4RSI
Yeast Smc2-Smc4 hinge domain with extended coiled coils
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.259, 49.707, 154.283 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 38.922 - 2.900 |
| Rwork | 0.223 |
| R-free | 0.26610 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.287 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 28485 |
| <I/σ(I)> | 33.5 |
| Completeness [%] | 90.2 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 16% PEG 300, 0.1 M Na/K phosphate pH 6.0, 8% glycerol, 10 mM dithiothreitol (DTT), VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
