4RS9

Structure of Myc3 N-terminal JAZ-binding domain [44-238] in complex with Jas motif of JAZ9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 32 2 1
Unit cell lengths	85.745, 85.745, 59.983
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.910 - 1.950
R-factor	0.20149
Rwork	0.200
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Myc3[44-238]
RMSD bond length	0.006
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.057
Number of reflections	18916
<I/σ(I)>	23.7
Completeness [%]	100.0	100
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M magnesium chloride, 0.1 M Tris, pH 8.5, and 30% (w/v) polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K