4RS7
Structure of pNOB8 ParB-C
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979, 0.980, 0.9252 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.210, 56.060, 72.300 |
| Unit cell angles | 90.00, 94.40, 90.00 |
Refinement procedure
| Resolution | 37.340 - 1.900 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.22430 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.711 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.340 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 59754 | |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG400, PEG8000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
