4RQW

Crystal structure of Myc3 N-terminal JAZ-binding domain [44-238] from Arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	57.254, 76.619, 85.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.930 - 2.200
R-factor	0.21688
Rwork	0.214
R-free	0.26285
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.272
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.050
Number of reflections	19794
<I/σ(I)>	20.6
Completeness [%]	99.9	100
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% (w/v) polyethylene glycol 3350, 0.2 M NaCl, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K