4RQO
Crystal structure of L-Serine Dehydratase from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.390, 81.390, 267.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.450 - 2.250 |
| R-factor | 0.20075 |
| Rwork | 0.198 |
| R-free | 0.25848 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.783 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.095 | 0.473 |
| Number of reflections | 49999 | |
| <I/σ(I)> | 37.8 | 3.6 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM Homopipes, 9-13% PEG-3400, 200 mM tetramethylammonium chloride, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
