4RQG
Crystal structure of Rhodostomin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.956, 34.179, 53.946 |
| Unit cell angles | 90.00, 119.26, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.660 |
| R-factor | 0.16268 |
| Rwork | 0.160 |
| R-free | 0.20786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uci |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.173 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 1.600 | 1.660 |
| Number of reflections | 17798 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 95.8 | 90.1 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 50mM MOPS, 32% PEG1500, 5% PEG200, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
