4RPG
Crystal structure of Micobacterium tuberculosis UDP-Galactopyranose mutase in complex with substrate UDP-Galp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.420, 99.470, 100.160 |
| Unit cell angles | 90.00, 110.81, 90.00 |
Refinement procedure
| Resolution | 47.270 - 2.400 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1VOJ |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.775 |
| Data reduction software | AutoProcess |
| Data scaling software | AutoProcess |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.094 | 0.764 |
| Total number of observations | 4478 | |
| Number of reflections | 61216 | |
| <I/σ(I)> | 9.68 | 1.6 |
| Completeness [%] | 98.9 | 98.8 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Hanging drop vapor diffusion | 5.5 | 277 | 0.1M Bis-Tris pH 5.5, 20% PEG 3350, 10mM Phenol, Hanging drop vapor diffusion, temperature 277K |
