4RPB

Crystal Structure of P Domain of Snow Mountain Norovirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-17
Detector	ADSC QUANTUM 315r
Spacegroup name	C 1 2 1
Unit cell lengths	166.640, 96.170, 64.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.660 - 1.610
R-factor	0.1659
Rwork	0.165
R-free	0.18160
Structure solution method	AB INITIO PHASING
RMSD bond length	0.005
RMSD bond angle	1.063
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ('(phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.660	1.640
High resolution limit [Å]	1.600	1.600
Rmerge		0.644
Number of reflections	254153
<I/σ(I)>		1.47
Completeness [%]	96.6	89.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	20% w/v PEG 2000, 0.1 M Tris pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K