4RP9

Bacterial vitamin C transporter UlaA/SgaT in C2 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.02245
Spacegroup name	C 1 2 1
Unit cell lengths	113.663, 85.812, 83.236
Unit cell angles	90.00, 127.97, 90.00

Refinement procedure

Resolution	32.791 - 1.651
R-factor	0.1358
Rwork	0.134
R-free	0.17400
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.386
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Number of reflections	71471
Completeness [%]	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1M MES pH 6.5, 0.1M NaCl, 30%(v/v) polyethyleneglycol(PEG)400, VAPOR DIFFUSION, HANGING DROP, temperature 291K