4RP7
Structure of the amyloid-forming segment TIITLE from p53 (residues 253-258)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 43.018, 4.849, 19.774 |
| Unit cell angles | 90.00, 92.12, 90.00 |
Refinement procedure
| Resolution | 21.494 - 1.576 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.19250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.861 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.494 | 1.650 |
| High resolution limit [Å] | 1.576 | 1.590 |
| Rmerge | 0.114 | 0.320 |
| Number of reflections | 636 | |
| <I/σ(I)> | 8.91 | 4.12 |
| Completeness [%] | 96.5 | 87.7 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | reservoir contained 0.01 M zinc chloride, 0.1 M MES buffer pH 6, and 20% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
