4RP4

Crystal Structure of the L27 domain of Discs Large 1 (target ID NYSGRC-010766) from Drosophila melanogaster (space group P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	77
Detector technology	CCD
Collection date	2013-03-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	47.958, 57.698, 64.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.210 - 1.420
R-factor	0.1505
Rwork	0.148
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rp3
RMSD bond length	0.022
RMSD bond angle	2.058
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.440
High resolution limit [Å]	1.420	3.850	1.420
Rmerge	0.095	0.078	0.934
Number of reflections	33288
<I/σ(I)>	15.9
Completeness [%]	95.9	87.6	94.5
Redundancy	15	13.7	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	0.2 M Sodium Format, 20 % PEG 3350, 3 % 1,8-Diaminooctane, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K