4RP3
Crystal Structure of the L27 Domain of Discs Large 1 (target ID NYSGRC-010766) from Drosophila melanogaster bound to a potassium ion (space group P212121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.979, 57.571, 64.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.360 |
| R-factor | 0.1501 |
| Rwork | 0.147 |
| R-free | 0.20560 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.013 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.380 |
| High resolution limit [Å] | 1.360 | 3.690 | 1.360 |
| Rmerge | 0.059 | 0.038 | 0.919 |
| Number of reflections | 39086 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 99.9 | 97.8 | 99.9 |
| Redundancy | 7.2 | 6.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Sodium Format, 20 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
