4ROX
Crystal Structure of P Domain of Hawaii Norovirus (GII.1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979961 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.060, 78.160, 74.660 |
| Unit cell angles | 90.00, 122.42, 90.00 |
Refinement procedure
| Resolution | 37.330 - 1.890 |
| R-factor | 0.1785 |
| Rwork | 0.177 |
| R-free | 0.20130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.892 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.330 | 37.330 | 1.910 |
| High resolution limit [Å] | 1.860 | 8.320 | 1.860 |
| Rmerge | 0.053 | 0.029 | 0.604 |
| Rmeas | 0.040 | 0.816 | |
| Number of reflections | 110624 | 1154 | 7974 |
| <I/σ(I)> | 8.92 | 21.7 | 1.31 |
| Completeness [%] | 98.9 | 91.4 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M KCl, 20% w/v PEG 3350, 2ul drops with 1:1 protein:precipitant ratio, VAPOR DIFFUSION, HANGING DROP, temperature 291K, vapor diffusion, hanging drop |
