4ROC
Human TFIIB-related factor 2 (Brf2) and TBP bound to U6#2 promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.968620 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.276, 89.911, 102.462 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.510 - 1.900 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.510 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 3.193 |
| Number of reflections | 56530 | |
| <I/σ(I)> | 11.2 | 0.5 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 10-20% PEG 3350, 50-100 mM MgCl2, 2 mM DTT, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
