4ROB
2.8A resolution structure of SRPN2 (K198C) from Anopheles gambiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.089, 167.210, 90.260 |
Unit cell angles | 90.00, 99.16, 90.00 |
Refinement procedure
Resolution | 41.803 - 2.800 |
R-factor | 0.2144 |
Rwork | 0.211 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pzf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.037 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER (2.5.1) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.250 | 47.250 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.163 | 0.051 | 0.530 |
Rpim | 0.031 | 0.351 | |
Total number of observations | 3698 | 15002 | |
Number of reflections | 31823 | ||
<I/σ(I)> | 5.9 | 16.3 | 1.9 |
Completeness [%] | 98.3 | 98.5 | 98.6 |
Redundancy | 3.2 | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 293 | 20% (w/v) PEG 8000, 100 Ches, pH 9.0, vapor diffusion, temperature 293K |