4ROA

1.90A resolution structure of SRPN2 (S358W) from Anopheles gambiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 63
Unit cell lengths	96.946, 96.946, 78.569
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.285 - 1.900
R-factor	0.1741
Rwork	0.172
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pzf
RMSD bond length	0.010
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	Aimless (0.2.8)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.470	48.470	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.079	0.041	0.798
Total number of observations		3166	18938
Number of reflections	33155
<I/σ(I)>	18.4	72	2.7
Completeness [%]	100.0	99.1	100
Redundancy	9.3	9.8	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	60% (w/v) Tacsimate, pH 7.0, vapor diffusion, temperature 293K