4RO9
2.0A resolution structure of SRPN2 (S358E) from Anopheles gambiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 171.329, 42.264, 185.623 |
| Unit cell angles | 90.00, 116.94, 90.00 |
Refinement procedure
| Resolution | 38.183 - 2.000 |
| R-factor | 0.1927 |
| Rwork | 0.190 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pzf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 93.150 | 93.150 | 2.040 |
| High resolution limit [Å] | 2.000 | 10.390 | 2.000 |
| Rmerge | 0.055 | 0.019 | 0.569 |
| Total number of observations | 1961 | 15190 | |
| Number of reflections | 80845 | ||
| <I/σ(I)> | 13.8 | 52.7 | 2.1 |
| Completeness [%] | 99.7 | 97.9 | 99.7 |
| Redundancy | 3.3 | 3.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 20% (w/v) PEG 3350, 100mM Bis-Tris Propane, 200 mM Sodium Malonate, pH 8.5, vapor diffusion, temperature 293K |
