4RO1
An 3'-5'-exoribonuclease that specifically recognizes RNAs.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.966, 54.201, 127.217 |
| Unit cell angles | 90.00, 107.09, 90.00 |
Refinement procedure
| Resolution | 40.532 - 2.803 |
| R-factor | 0.2697 |
| Rwork | 0.269 |
| R-free | 0.29360 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 121.600 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 37850 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% PEG 4000, 0.15M (NH4)2SO4, 0.1M HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
