4RNT

HIS 92 ALA MUTATION IN RIBONUCLEASE T1 INDUCES SEGMENTAL FLEXIBILITY. AN X-RAY STUDY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	31.040, 62.310, 43.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.158 *
RMSD bond length	0.021
RMSD bond angle	0.075
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]		2.200 *
Rmerge	0.072 *
Total number of observations	6189 *
Number of reflections	982 *
Completeness [%]		69 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (%)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	PEG4000	30 (%(w/v))