4RNS
PcpR inducer binding domain (apo-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | H 3 |
Unit cell lengths | 169.450, 169.450, 109.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.362 - 2.700 |
R-factor | 0.1624 |
Rwork | 0.161 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y7w |
RMSD bond length | 0.010 |
RMSD bond angle | 1.092 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.520 | 2.800 |
High resolution limit [Å] | 2.700 | 3.320 | 2.700 |
Rmerge | 0.139 | ||
Number of reflections | 57389 | ||
Completeness [%] | 98.6 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M sodium tartrate dibasic dihydrate, 20% w/v polyethylene glycol 3,350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |