4RNL
The crystal structure of a possible galactose mutarotase from Streptomyces platensis subsp. rosaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.425, 115.800, 261.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.333 - 1.800 |
| R-factor | 0.1561 |
| Rwork | 0.154 |
| R-free | 0.19190 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.064 |
| Data reduction software | DENZO (Builder/HKL3000) |
| Data scaling software | SCALEPACK (Builder/HKL3000) |
| Phasing software | SHELXD (Builder/HKL3000) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.137 | 0.765 |
| Number of reflections | 135006 | |
| <I/σ(I)> | 16.6 | 2.52 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 1M Ammonium phospahte Dibasic, 0.1M Sodium acetate: acetate acid, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
